AB0050 A NOVEL METHOD FOR ISOLATION OF EXOSOMES FROM SYNOVIAL FLUID

Background: Exosomes in synovial fluid (SF) has a close relationship with the pathogenesis of rheumatiod arthritis. As complex biological fluid, SF presents challenges for exosomes isolation using standard methods, such as Exoquick TM kit and ultracentrifugation. Objectives: The study aims to compared quality separated by (TM), kit+Exoquick TC (TM-TC), ultracentrifugation (UC) TM-TC+UC(TM-TC-UC) from SF. Methods: was TM, TM-TC, UC TM-TC-UC respectively. size concentrations were detected high sensitivity flow cytometry nanoparticle analysis. Total protein RNA extracted exosomes. SDS-PAGE used detect distribution Western blot examine level albumin marker (TSG101 CD81). Results: There no statistic difference diameters four methods. (5.65±0.93), (3.02±1.19), (1.67±0.25) (4.61±0.73) *10 9 Particles/mL. methods consistent showed that different. Low levels TM-TC TM-TC-UC, while UC. higher than other groups. Conclusion: can be obtain exosome-enriched components. References: [1]Helwa I, et al, A Comparative Study Serum Exosome Isolation Using Differential Ultracentrifugation Three Commercial Reagents. PloS one, 2017. 12(1): p. e0170628-e0170628. Figure 1. : exosomes; B detection albumin, TSG101 CD81 western blot. Disclosure Interests: None declared